Download Program
Lecturers
- Upinder Bhalla National Centre for Biological Sciences, India
- Juan Burrone King's College London, UK
- Santiago Canals CSIC-UMH, Spain
- Sophie Deneve Ecole Normale Superieure, France
- Michael Dickinson Caltech, Pasadena, USA
- Florian Engert Harvard University, USA
- Michale Fee Massachusetts Institute of Technology, USA
- Balázs Hangya IEM, Hungarian Academy of Sciences, Hungary
- Sonja Hofer Biozentrum, University of Basel, Switzerland
- Tomα Hromαdka Slovak Academy of Sciences, Slovakia
- Benjamin Judkewitz Einstein Center for Neuroscience, Germany
- Georg Keller Friedrich Miescher Institute, Switzerland
- Suhasa Kodandaramaiah University of Minnesota, USA
- Christian Machens - Champalimaud Foundation, Portugal
- Hannah Monyer DKFZ, University of Heidelberg, Germany
- Tom Mrsic-Flögel Sainsbury Wellcome Centre, University College London, UK
- Ruben Portugues Max Planck Institute of Neurobiology, Germany
- Tobias Rose Max Planck Institute of Neurobiology, Germany
- Botond Roska Friedrich Miescher Institute, Switzerland
- Wolf Singer Max Planck Institute for Brain Research, Germany
- Marcus Stephenson Jones Sainsbury Wellcome Centre, UCL, UK
- Nao Uchida Harvard University, USA
- Chris Xu Cornell University, USA
- Tony Zador Cold Spring Harbor Laboratory, USA
Organizers
- Florin Albeanu Cold Spring Harbor Laboratory, NY, USA
- Adam Kampff Sainsbury Wellcome Centre, University College London, UK
- Raul Mureşan Transylvanian Institute of Neuroscience, Cluj-Napoca, Romania
Teaching Assistants
- Antonin Blot Sainsbury Wellcome Centre, UCL, UK
- Rob Campbell Sainsbury Wellcome Centre, UCL, UK
- Federico Carnevale Cold Spring Harbor Laboratory, NY, USA
- Adriana Nagy-Dăbâcan Transylvanian Institute of Neuroscience, Cluj-Napoca, Romania
- Medorian Gheorghiu Transylvanian Institute of Neuroscience, Cluj-Napoca, Romania
- Priyanka Gupta National Centre for Biological Sciences, Bangalore, India & CSHL, NY, USA
- Mitra Javadzadeh Sainsbury Wellcome Centre, UCL, UK
- Gonçalo Lopes Champalimaud Foundation, Centre for the Unknown, Lisbon, Portugal
- Fred Marbach Cold Spring Harbor Laboratory, NY, USA
- Vasile Moca Transylvanian Institute of Neuroscience, Cluj-Napoca, Romania
- Mehrab N. Modi Cold Spring Harbor Laboratory, NY, USA
- Jon Newman Massachusetts Institute of Technology, USA
- Bruno Pichler INSS (Independent NeuroScience Services)
- Nacho Sanguinetti Bernstein Center for Computational Neuroscience, Berlin, Germany
- Iuliu Vasilescu Politechnica University, Bucharest, Romania
- Jakob Voigts Massachusetts Institute of Technology, USA
- Petr Znamenskiy Sainsbury Wellcome Centre, UCL, UK
Topics
- Basic Optics Diffraction and Resolution. Illumination Techniques. Numerical Aperture.
- Optical bench exercises Lenses, optical systems, illumination methods, basic microscopy techniques. How to custom build different kinds of microscopes.
- Noise measurements and photo-sensors Shot noise, optical detectors, amplifiers, NI-DAQ, CCD cameras, photodiodes, photo multiplier tubes (PMTs).
- Light and fluorescence microscopy Fluorescence, FRAP, photo-activation, photo-conversion. Point spread function measurements, basic image analysis (deconvolution, denoising, PCA).
- Fluorescence probes GFP, GFP based chromophores, organic calcium dyes, genetically encoded calcium dyes, pHluorins, voltage sensitive dyes.
- Intrinsic Optical Imaging Visual, auditory & barrel cortex; olfactory bulb. Students will build a custom wide field fluorescence and intrinsic optical imaging rig.
- Scanning microscopy Confocal and two-photon microscopy. Lasers. Students will build a two-photon microscope and write custom scanning and acquisition software in MATLAB and NI DAQmx. The ScanImage API.
- Viral approaches to label, monitor and alter neuronal circuits.
- Optogenetics Light activated ion channels and pumps. Patterned photo-stimulation techniques.
- Benchtop electronics and basic electrophysiology Impedence and Dipoles. Amplifiers. Extracellular and intracellular recordings. LFP; single unit, multi-unit extracellular recordings, tetrodes, electrode arrays; patch clamp.
- Awake head fixed and freely moving optical and electrophysiological recording strategies in rodents Microdrives. Fiber optic based systems. Open source systems. Open Ephys.
- Techniques for electrophysiological data analysis.
- Monitoring animal behavior Open Source tools for acquisition and analysis of video data. Intro to Bonsai and Arduino. Training Strategies. Closed loop systems.
- Neuronal functional connectivity and neuronal connectomics Serial electron-microscopy and trans-synaptic labeling methods.
- Synchrony and oscillations.
- Cortical attention, sparse neuronal codes.
- Decision making, uncertainty, neuro-modulatory systems.
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