Download Program
Confirmed Lecturers
- Upinder Bhalla – National Centre for Biological Sciences, Bangalore, India
- Florian Engert – Harvard University, Cambridge, MA, USA
- Winrich Freiwald – The Rockefeller University, New York, NY, USA
- Balázs Hangya – Institute of Experimental Medicine, Hungarian Academy of Sciences, Hungary
- Hofer, Sonja – Biozentrum, University of Basel, Switzerland
- Tomáš Hromádka – Institute of Neuroimmunology, Slovak Academy of Sciences, Bratislava, Slovakia
- Mark Hübener – Max Planck Institute of Neurobiology, Martinsried, Germany
- Georg Keller – Friedrich Miescher Institute, Basel, Switzerland
- David Kleinfeld – University of California, San Diego, USA
- Suhasa Kodandaramaiah – University of Minnesota, MN, USA
- Liora Las – Weizmann Institute of Science, Rehovot, Israel
- Hannah Monyer – University of Heidelberg, Germany
- Tom Mrsic-Flögel – Biozentrum, University of Basel, Switzerland
- Venkatesh Murthy – Harvard University, Cambridge, MA, USA
- Ruben Portugues – Max Planck Institute of Neurobiology, Martinsried, Germany
- Botond Roska – Friedrich Miescher Institute, Basel, Switzerland
- Marcus Stephenson Jones – Sainsbury Wellcome Centre, University College London, UK
- Wolf Singer – Max Planck Institute for Brain Research, Frankfurt, Germany
- Chris Xu – Cornell University, USA
- Tony Zador – Cold Spring Harbor Laboratory, NY, USA
Organizers
- Raul Mureşan – Romanian Institute of Science and Technology, Cluj-Napoca, Romania
- Adam Kampff – Sainsbury Wellcome Centre, University College London, UK
- Florin Albeanu – Cold Spring Harbor Laboratory, NY, USA
Teaching Assistants
- Rob Campbell – Biozentrum, University of Basel, Switzerland
- Federico Carnevale – Cold Spring Harbor Laboratory, NY, USA
- Adriana Dăbâcan – Romanian Institute of Science and Technology, Cluj-Napoca, Romania
- Medorian Gheorghiu – Romanian Institute of Science and Technology, Cluj-Napoca, Romania
- Priyanka Gupta – National Centre for Biological Sciences, Bangalore, India & CSHL, NY, USA
- Mitra Javadzadeh – Biozentrum, University of Basel, Switzerland
- Marcus Leinweber – Friedrich Miescher Institute, Basel, Switzerland
- Gonçalo Lopes – Champalimaud Foundation, Centre for the Unknown, Lisbon, Portugal
- Fred Marbach – Cold Spring Harbor Laboratory, NY, USA
- Vasile Moca – Romanian Institute of Science and Technology, Cluj-Napoca, Romania
- Jon Newman – Massachusetts Institute of Technology, USA
- Marius Pachiţariu – University College London, London, UK
- Bruno Pichler – Independent NeuroScience Services (INSS), UK
- Nacho Sanguinetti – Bernstein Center for Computational Neuroscience, Berlin, Germany
- Jakob Voigts – Massachusetts Institute of Technology, USA
- Petr Znamenskiy – Biozentrum, University of Basel, Switzerland
Topics
- Basic Optics – Diffraction and Resolution. Illumination Techniques. Numerical Aperture.
- Optical bench exercises – Lenses, optical systems, illumination methods, basic microscopy techniques. How to custom build different kinds of microscopes.
- Noise measurements and photo-sensors – Shot noise, optical detectors, amplifiers, NI-DAQ, CCD cameras, photodiodes, photo multiplier tubes (PMTs).
- Light and fluorescence microscopy – Fluorescence, FRAP, photo-activation, photo-conversion. Point spread function measurements, basic image analysis (deconvolution, denoising, PCA).
- Fluorescence probes – GFP, GFP based chromophores, organic calcium dyes, genetically encoded calcium dyes, pHluorins, voltage sensitive dyes.
- Intrinsic Optical Imaging – Visual, auditory & barrel cortex; olfactory bulb. Students will build a custom wide field fluorescence and intrinsic optical imaging rig.
- Scanning microscopy – Confocal and two-photon microscopy. LASERs. Introduction to Labview. Students will build a two-photon microscope and write custom scanning and acquisition software in Labiew. Open source systems.
- Viral approaches to label, monitor and alter neuronal circuits.
- Optogenetics – Light activated ion channels and pumps. Patterned photo-stimulation techniques.
- Benchtop electronics and basic electrophysiology – Impedence and Dipoles. Amplifiers. Extracellular and intracellular recordings. LFP; single unit, multi-unit extracellular recordings, tetrodes, electrode arrays; patch clamp.
- Awake head fixed and freely moving optical and electrophysiological recording strategies in rodents – Microdrives. Fiber optic based systems. Open source systems.
- Techniques for electrophysiological data analysis.
- Monitoring animal behavior – Open Source tools for acquisition and analysis of video data. Intro to Python and Arduino. Training Strategies. Closed loop systems.
- Neuronal functional connectivity and neuronal connectomics – Serial electron-microscopy and trans-synaptic labeling methods.
- Synchrony and oscillations.
- Cortical attention, sparse neuronal codes.
- Decision making, uncertainty, neuro-modulatory systems.
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