Download Program


Confirmed Lecturers

  • Albeanu, Florin – Cold Spring Harbor Laboratory, NY, USA
  • Bhalla, Upinder – National Centre for Biological Sciences, Bangalore, India
  • Burrone, Juan – King’s College, London, UK
  • Engert, Florian – Harvard University, Cambridge, MA, USA
  • Gradinaru, Viviana – California Institute of Technology, Pasadena, CA, USA
  • Hofer, Sonja – Biozentrum, University of Basel, Switzerland
  • Hromαdka, Tomᚠ– Institute of Experimental Medicine, Prague, Czech Republic
  • Hübener, Mark – Max Planck Institute of Neurobiology, Martinsried, Germany
  • Kampff, Adam – Champalimaud Foundation, Institute for the Unknown, Lisbon, Portugal
  • Katzner, Steffen – Centre for Integrative Neuroscience, University of Tübingen, Germany
  • Keller, Georg – Friedrich Miescher Institute, Basel, Switzerland
  • Kepecs, Adam – Cold Spring Harbor Laboratory, NY, USA
  • Monyer, Hannah – University of Heidelberg, Germany
  • Mrsic-Flögel, Tom – Biozentrum, University of Basel, Switzerland
  • Mureşan, Raul – Coneural, Romanian Institute of Science and Technology, Cluj-Napoca, Romania
  • Murthy, Venkatesh – Harvard University, Cambridge, MA, USA
  • Nikolić, Danko – Max Planck Institute for Brain Research, Frankfurt, Germany
  • Ölveczky, Bence – Harvard University, Cambridge, MA, USA
  • Shoham, Shy – The Technion - Israel Institute of Technology, Haifa, Israel
  • Singer, Wolf – Max Planck Institute for Brain Research, Frankfurt, Germany

Organizers

  • Mureşan, Raul – Coneural, Romanian Institute of Science and Technology, Cluj-Napoca, Romania
  • Kampff, Adam – Champalimaud Foundation, Institute for the Unknown, Lisbon, Portugal
  • Albeanu, Florin – Cold Spring Harbor Laboratory, NY, USA

Teaching Assistants

  • Dăbâcan, Adriana – Coneural, Romanian Institute of Science and Technology, Cluj-Napoca, Romania
  • Dhawale, Ashesh – Harvard University, Cambridge, MA, USA
  • Dreosti, Elena – University College London, UK
  • Gupta, Priyanka – National Centre for Biological Sciences, Bangalore, India & CSHL, NY, USA
  • Hangya, Balázs – Cold Spring Harbor Laboratory, NY, USA
  • Jurjuţ, Ovidiu – Centre for Integrative Neuroscience, Tübingen, Germany
  • Moca, Vasile – Coneural, Romanian Institute of Science and Technology, Cluj-Napoca, Romania
  • Modi, Mehrab N. – National Centre for Biological Sciences, Bangalore, India
  • Siegle, Josh – Massachusetts Institute of Technology, Cambridge, MA, USA
  • Ţincaş, Ioana – Coneural, Romanian Institute of Science and Technology, Cluj-Napoca, Romania
  • Znamenskiy, Petr – Biozentrum, University of Basel, Switzerland

Topics

  • Basic Optics – Diffraction and Resolution. Illumination Techniques. Numerical Aperture.
  • Optical bench exercises – Lenses, optical systems, illumination methods, basic microscopy techniques. How to custom build different kinds of microscopes.
  • Noise measurements and photo-sensors – Shot noise, optical detectors, amplifiers, NI-DAQ, CCD cameras, photodiodes, photo multiplier tubes (PMTs).
  • Light and fluorescence microscopy – Fluorescence, FRAP, photo-activation, photo-conversion. Point spread function measurements, basic image analysis (deconvolution, denoising, PCA).
  • Fluorescence probes – GFP, GFP based chromophores, organic calcium dyes, genetically encoded calcium dyes, pHluorins, voltage sensitive dyes.
  • Intrinsic Optical Imaging – Visual, auditory & barrel cortex; olfactory bulb. Students will build a custom wide field fluorescence and intrinsic optical imaging rig.
  • Scanning microscopy – Confocal and two-photon microscopy. LASERs. Introduction to Labview. Students will build a two-photon microscope and write custom scanning and acquisition software in Labiew. Open source systems.
  • Viral approaches to label, monitor and alter neuronal circuits.
  • Optogenetics – Light activated ion channels and pumps. Patterned photo-stimulation techniques.
  • Benchtop electronics and basic electrophysiology – Impedence and Dipoles. Amplifiers. Extracellular and intracellular recordings. LFP; single unit, multi-unit extracellular recordings, tetrodes, electrode arrays; patch clamp.
  • Awake head fixed and freely moving optical and electrophysiological recording strategies in rodents – Microdrives. Fiber optic based systems. Open source systems.
  • Techniques for electrophysiological data analysis.
  • Monitoring animal behavior – Open Source tools for acquisition and analysis of video data. Intro to Python and Arduino. Training Strategies. Closed loop systems.
  • Neuronal functional connectivity and neuronal connectomics – Serial electron-microscopy and trans-synaptic labeling methods.
  • Synchrony and oscillations.
  • Cortical attention, sparse neuronal codes.
  • Decision making, uncertainty, neuro-modulatory systems.
  • Introduction to theoretical and computational modeling of neuronal circuits.

© TENSS 2014