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Confirmed Lecturers
- Albeanu, Florin Cold Spring Harbor Laboratory, NY, USA
- Bhalla, Upinder National Centre for Biological Sciences, Bangalore, India
- Burrone, Juan Kings College, London, UK
- Engert, Florian Harvard University, Cambridge, MA, USA
- Gradinaru, Viviana California Institute of Technology, Pasadena, CA, USA
- Hofer, Sonja Biozentrum, University of Basel, Switzerland
- Hromαdka, Tomα Institute of Experimental Medicine, Prague, Czech Republic
- Hübener, Mark Max Planck Institute of Neurobiology, Martinsried, Germany
- Kampff, Adam Champalimaud Foundation, Institute for the Unknown, Lisbon, Portugal
- Katzner, Steffen Centre for Integrative Neuroscience, University of Tübingen, Germany
- Keller, Georg Friedrich Miescher Institute, Basel, Switzerland
- Kepecs, Adam Cold Spring Harbor Laboratory, NY, USA
- Monyer, Hannah University of Heidelberg, Germany
- Mrsic-Flögel, Tom Biozentrum, University of Basel, Switzerland
- Mureşan, Raul Coneural, Romanian Institute of Science and Technology, Cluj-Napoca, Romania
- Murthy, Venkatesh Harvard University, Cambridge, MA, USA
- Nikolić, Danko Max Planck Institute for Brain Research, Frankfurt, Germany
- Ölveczky, Bence Harvard University, Cambridge, MA, USA
- Shoham, Shy The Technion - Israel Institute of Technology, Haifa, Israel
- Singer, Wolf Max Planck Institute for Brain Research, Frankfurt, Germany
Organizers
- Mureşan, Raul Coneural, Romanian Institute of Science and Technology, Cluj-Napoca, Romania
- Kampff, Adam Champalimaud Foundation, Institute for the Unknown, Lisbon, Portugal
- Albeanu, Florin Cold Spring Harbor Laboratory, NY, USA
Teaching Assistants
- Dăbâcan, Adriana Coneural, Romanian Institute of Science and Technology, Cluj-Napoca, Romania
- Dhawale, Ashesh Harvard University, Cambridge, MA, USA
- Dreosti, Elena University College London, UK
- Gupta, Priyanka National Centre for Biological Sciences, Bangalore, India & CSHL, NY, USA
- Hangya, Balázs Cold Spring Harbor Laboratory, NY, USA
- Jurjuţ, Ovidiu Centre for Integrative Neuroscience, Tübingen, Germany
- Moca, Vasile Coneural, Romanian Institute of Science and Technology, Cluj-Napoca, Romania
- Modi, Mehrab N. National Centre for Biological Sciences, Bangalore, India
- Siegle, Josh Massachusetts Institute of Technology, Cambridge, MA, USA
- Ţincaş, Ioana Coneural, Romanian Institute of Science and Technology, Cluj-Napoca, Romania
- Znamenskiy, Petr Biozentrum, University of Basel, Switzerland
Topics
- Basic Optics Diffraction and Resolution. Illumination Techniques. Numerical Aperture.
- Optical bench exercises Lenses, optical systems, illumination methods, basic microscopy techniques. How to custom build different kinds of microscopes.
- Noise measurements and photo-sensors Shot noise, optical detectors, amplifiers, NI-DAQ, CCD cameras, photodiodes, photo multiplier tubes (PMTs).
- Light and fluorescence microscopy Fluorescence, FRAP, photo-activation, photo-conversion. Point spread function measurements, basic image analysis (deconvolution, denoising, PCA).
- Fluorescence probes GFP, GFP based chromophores, organic calcium dyes, genetically encoded calcium dyes, pHluorins, voltage sensitive dyes.
- Intrinsic Optical Imaging Visual, auditory & barrel cortex; olfactory bulb. Students will build a custom wide field fluorescence and intrinsic optical imaging rig.
- Scanning microscopy Confocal and two-photon microscopy. LASERs. Introduction to Labview. Students will build a two-photon microscope and write custom scanning and acquisition software in Labiew. Open source systems.
- Viral approaches to label, monitor and alter neuronal circuits.
- Optogenetics Light activated ion channels and pumps. Patterned photo-stimulation techniques.
- Benchtop electronics and basic electrophysiology Impedence and Dipoles. Amplifiers. Extracellular and intracellular recordings. LFP; single unit, multi-unit extracellular recordings, tetrodes, electrode arrays; patch clamp.
- Awake head fixed and freely moving optical and electrophysiological recording strategies in rodents Microdrives. Fiber optic based systems. Open source systems.
- Techniques for electrophysiological data analysis.
- Monitoring animal behavior Open Source tools for acquisition and analysis of video data. Intro to Python and Arduino. Training Strategies. Closed loop systems.
- Neuronal functional connectivity and neuronal connectomics Serial electron-microscopy and trans-synaptic labeling methods.
- Synchrony and oscillations.
- Cortical attention, sparse neuronal codes.
- Decision making, uncertainty, neuro-modulatory systems.
- Introduction to theoretical and computational modeling of neuronal circuits.
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