Download Program


Confirmed Lecturers

  • Upinder Bhalla – National Centre for Biological Sciences, Bangalore, India
  • Juan Burrone – King’s College, London, UK
  • Michael Dickinson – Caltech, Pasadena, CA, USA
  • Florian Engert – Harvard University, Cambridge, MA, USA
  • Winrich Freiwald – The Rockefeller University, New York, NY, USA
  • Tamás Freund – Institute of Experimental Medicine, Hungarian Academy of Sciences, Hungary
  • TomᚠHromαdka – Institute of Neuroimmunology, Slovak Academy of Sciences, Bratislava, Slovakia
  • Mark Hübener – Max Planck Institute of Neurobiology, Martinsried, Germany
  • Balázs Hangya – Institute of Experimental Medicine, Hungarian Academy of Sciences, Hungary
  • Na Ji – Howard Hughes Medical Institute, Janelia Farm, Ashburn, VA, USA
  • Georg Keller – Friedrich Miescher Institute, Basel, Switzerland
  • Suhasa Kodandaramaiah – University of Minnesota, MN, USA
  • Hannah Monyer – University of Heidelberg, Germany
  • Tom Mrsic-Flögel – Biozentrum, University of Basel, Switzerland
  • Venkatesh Murthy – Harvard University, Cambridge, MA, USA
  • Danko Nikolić – Max Planck Institute for Brain Research, Frankfurt, Germany
  • Ruben Portugues – Max Planck Institute of Neurobiology, Martinsried, Germany
  • Wolf Singer – Max Planck Institute for Brain Research, Frankfurt, Germany
  • Karel Svoboda – Howard Hughes Medical Institute, Janelia Farm, Ashburn, VA, USA
  • James Watson – Cold Spring Harbor Laboratory, NY, USA
  • Tony Zador – Cold Spring Harbor Laboratory, NY, USA

Organizers

  • Raul Mureşan – Romanian Institute of Science and Technology, Cluj-Napoca, Romania
  • Adam Kampff – Sainsbury Wellcome Centre, University College London, UK
  • Florin Albeanu – Cold Spring Harbor Laboratory, NY, USA

Teaching Assistants

  • Arkarup Banerjee – Cold Spring Harbor Laboratory, NY, USA
  • Rob Campbell – Biozentrum, University of Basel, Switzerland
  • Federico Carnevale – Cold Spring Harbor Laboratory, NY, USA
  • Adriana Dăbâcan – Romanian Institute of Science and Technology, Cluj-Napoca, Romania
  • Medorian Gheorghiu – Romanian Institute of Science and Technology, Cluj-Napoca, Romania
  • Priyanka Gupta – National Centre for Biological Sciences, Bangalore, India & CSHL, NY, USA
  • Ovidiu Jurjut – Centre for Integrative Neuroscience, Tübingen, Germany
  • Gonçalo Lopes – Champalimaud Foundation, Centre for the Unknown, Lisbon, Portugal
  • Vasile Moca – Romanian Institute of Science and Technology, Cluj-Napoca, Romania
  • Mehrab N. Modi – Cold Spring Harbor Laboratory, NY, USA
  • Ivana Orsolic – Biozentrum, University of Basel, Switzerland
  • Marius Pachiţariu – University College London, London, UK
  • Nacho Sanguinetti – Bernstein Center for Computational Neuroscience, Berlin, Germany
  • Josh Siegle – Allen Institute for Brain Science, Seattle, WA, USA
  • Petr Znamenskiy – Biozentrum, University of Basel, Switzerland

Topics

  • Basic Optics – Diffraction and Resolution. Illumination Techniques. Numerical Aperture.
  • Optical bench exercises – Lenses, optical systems, illumination methods, basic microscopy techniques. How to custom build different kinds of microscopes.
  • Noise measurements and photo-sensors – Shot noise, optical detectors, amplifiers, NI-DAQ, CCD cameras, photodiodes, photo multiplier tubes (PMTs).
  • Light and fluorescence microscopy – Fluorescence, FRAP, photo-activation, photo-conversion. Point spread function measurements, basic image analysis (deconvolution, denoising, PCA).
  • Fluorescence probes – GFP, GFP based chromophores, organic calcium dyes, genetically encoded calcium dyes, pHluorins, voltage sensitive dyes.
  • Intrinsic Optical Imaging – Visual, auditory & barrel cortex; olfactory bulb. Students will build a custom wide field fluorescence and intrinsic optical imaging rig.
  • Scanning microscopy – Confocal and two-photon microscopy. LASERs. Introduction to Labview. Students will build a two-photon microscope and write custom scanning and acquisition software in Labiew. Open source systems.
  • Viral approaches to label, monitor and alter neuronal circuits.
  • Optogenetics – Light activated ion channels and pumps. Patterned photo-stimulation techniques.
  • Benchtop electronics and basic electrophysiology – Impedence and Dipoles. Amplifiers. Extracellular and intracellular recordings. LFP; single unit, multi-unit extracellular recordings, tetrodes, electrode arrays; patch clamp.
  • Awake head fixed and freely moving optical and electrophysiological recording strategies in rodents – Microdrives. Fiber optic based systems. Open source systems.
  • Techniques for electrophysiological data analysis.
  • Monitoring animal behavior – Open Source tools for acquisition and analysis of video data. Intro to Python and Arduino. Training Strategies. Closed loop systems.
  • Neuronal functional connectivity and neuronal connectomics – Serial electron-microscopy and trans-synaptic labeling methods.
  • Synchrony and oscillations.
  • Cortical attention, sparse neuronal codes.
  • Decision making, uncertainty, neuro-modulatory systems.

© TENSS 2016